Angularly scanned Mach–Zehnder inverse-scattering microscope
Reconstructs the acquisition geometry in Figure 2a of Kim et al.'s bioRxiv v1: a 635 nm fiber-delivered CW source is collimated at L1; BS1 separates a scanned sample arm and reference arm; a dual-axis galvo changes the sample illumination from on-axis at its centered command to off-axis across its angular sweep; matched 200 mm L2/L3 relays drive matched 16× water-dipping objectives (NA 0.8, WD 3.0 mm) around the sample; and BS2 sends the sample beam and a reference beam with a small diagrammatic tilt onto the same CMOS sensor. That fixed camera-side crossing angle represents the carrier used for off-axis digital holography and is distinct from the galvo-controlled illumination angle; its drawn magnitude is illustrative because the paper does not report a value. The scene instantiates the paper's long-working-distance objective variant used for thick organoids and zebrafish. The Methods also document a matched 60× oil, NA 1.42 high-resolution alternative. OpticalSetup traces qualitative ray paths and the galvo sweep, but it does not simulate coherent phase, interferograms, multiple scattering, digital defocus, or MSBP reconstruction.
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